ePub Quantitation of mRNA By Polymerase Chain Reaction ePub

by Thomas Köhler

In this laboratory "cook-book," the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

eBook tags: quantitation, mrna, polymerase, chain, reaction, epub, download, epub, thomas köhler

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ISBN
Book Title
Book Author
PublisherSpringer
Release date 02.11.1995
Pages count166
eBook formatHardcover, (torrent)En
File size1.4 Mb
Book rating0 (0 votes)
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